Resistin and adiponectine secretion pattern after GnRH stimulus in patients with functional hypothalamic amenorrhea.
Adam Czyzyk (PL), Aleksandra Szuflak (PL), Agnieszka Podfigurna (PL), Kinga Polak (IT), Dorota Filipowicz (PL), Blazej Meczekalski (PL)
[Czyzyk] Poznan University of Medical Sciences, Department of Gynaecological Endocrinology, [Szuflak] Poznan University of Medical Sciences, Students scientific association at the Department of Gynaecological Endocrinology, [Podfigurna] Poznan University of Medical Sciences, Department of Gynaecological Endocrinology, [Polak] Department of Clinical and Experimental Medicine, Division of Obstetrics and Gynecology, University of Pisa, Pisa, Italy., [Filipowicz] Poznan University of Medical Sciences, Students scientific association at the Department of Gynaecological Endocrinology, [Meczekalski] Poznan University of Medical Sciences, Department of Gynaecological Endocrinology
Context Patients diagnosed with FHA, presents complex of endocrine and metabolic disturbances which are consequences of disturbed GnRH hypothalamic secretion. Some patients with secondary amenorrhea are believed to represent both: FHA and PCOS pathologies. Adiponectin (A) functions as anti-inflammatory, anti-atherogenic, cardioprotective, and insulin-sensitizing hormone. Resistin (R) is positively linked with inflammation, cardiovascular risk, obesity and IR. Objective The aim of the study was to assess the episodic secretion of A and R in FHApatients FHA before and after exogenous GnRH stimuli. Methods The following parameters were available for each participant: age, body mass index (BMI), blood pressure, menstrual history, hormonal data (FSH, LH, estradiol, testosterone [T], DHEAS) and metabolic data (fasting glucose and insulin, lipid profile). Patients 13 women (aged 24 +/-3) with FHA Interventions GnRH stimulation test with blood withdrawal according to the schedule: 0’, 15’ 30’ 45’ 60’ 90’ with Main Outcome Measures FSH, LH, A, R serum concentartions. Results All patients responded with increase of LH and FSH secretion after GnRH. We divided them depending on concentration of LH after 30’ to groups: A – with increase <15x of the basic concentration and group B – with increase >=15x. Group A showed lower mean basic concentrations of A and R in comparison to group B. Moreover in group A we found a significant decrease of A after 30’ and R after 45’ and from GnRH injection, in comparison to basic concentrations. We failed to found any specific pattern of A and R secretion in group B. There was positive correlation between FSH and A/R ratio in 60’ of GnRH stimulation test. Conclusions Measuring A and R after GnRH injection in patients with secondary amenorrhea potentially may differentiate FHA patients from those with FHA/PCOS overlapping syndrome.