Correlation between signaling pathways activation level in eutopic and ectopic endometrium.
Yana Aznaurova (RU), Andrew Garazha (RU), Anton Buzdin (RU), Leila Adamyan (RU)
[Aznaurova] A.I.Evdokimov Moscow State Medical & Dental University, [Garazha] First Oncology Research and Advisory Center, [Buzdin] First Oncology Research and Advisory Center, [Adamyan] A.I.Evdokimov Moscow State Medical & Dental University
Context: The absence of qualitative non-invasive methods of timely diagnosis of endometriosis forms the relevance of studying this disease at molecular level. Objective:to identify all specific changes of intracellular signaling pathways (ISP) activity in eu- and ectopic endometrium and the correlation of molecular abnormalities in these locations. Methods: transcriptome of cells has been investigated by microchip hybridization (CustomArray). The profiles of ISP activation in the studied samples were calculated using bioinformatics methods. Patients:20 women with different forms of endometriosis, who were not treated with hormonal medicines before surgery. We have fully analyzed data of 10 patients (10 eu-, 10 ectopic and 5 normal endometrial samples). Intervention: laparoscopic endometriosis excision of ectopic foci, hysteroscopy with endometrial scraping. After that RNA was isolated from the obtained tissue samples for further transcriptome analysis. Main Outcome Measures: we have identified the top-15 differentially up- and down-activated ISP in endometriosis foci compared to the normal endometrium. Firstly we normalized all pathway activation profiles on the normal endometrium sample and then compare eu- and ectopic endometrium with each other. Results: estimation of obtained data identified 85% correlation in molecular changes between eutopic endometrium and right ovarian endometriotic cyst. Almost 98% correlation was found between eutopic endometrium and peritoneal endometriosis. Conclusion: evaluation of changes in the eutopic endometrium may serve as a marker of tissue condition in ectopic foci. The analysis of ISP activity in eutopic endometrium can be used as a reliable monitoring method of the effectiveness of the therapy. It will be possible to track the status of ectopic endometrium by analyzing eutopic endometrium (hysteroscopy versus relaparoscopy).