Evaluation of fluorescent rapid diagnosis of the endometrium in the complex preparation for pregnancy and program IVF.
Vladimir Zuev (RU), Michail Alexandrov (RU), Elena Kalinina (RU), Anatoly Ischenko (RU), Tea Dzhibladze (RU), Irina Khokhlova (RU), Yulia Pimancheva (RU)
[Zuev] 1st medical university of moscow, [Alexandrov] , [Kalinina] , [Ischenko] , [Dzhibladze] , [Khokhlova] , [Pimancheva]
«Thin» endometrium prevents implantation and pregnancy. Aim of the study: To evaluate the capacity and efficiency of Raman-fluorescent conversion diagnostic technology in women with failed IVF programs. Methods. Raman-spectral conversion diagnostic, ultrasonography. Office hysteroscopy, morphologic and immunochemical methods. Spectral features (levels of reflection and luminescence) were measured in 3 areas –top part, middle and internal mouth of the uterus. Point to compare was skin point in internal part of femur. Raman-fluorescence was used to determine oxygenation, proliferation, microcirculation and aerobic or anaerobic metabolism in endometrium «on line». Non- invasive procedure. Time for detection – 1 minute.> Patients.340 patients age of 32-47 years with noneffective (2-12) IVF programs. In 315 (92,6%) women chronic endometritis was seen by morphologic evaluation. In 25 (7,4%) – hormonal disorders. Results. Raman-fluorescence technology enabled to determine oxygenation, proliferation, microcirculation and aerobic/anaerobic/metabolism in endometrium. Laser beam can delivered to endometrium by flexible guide. Fluorescence of normal endometrium showed to be 1,2-1,7 Ralative units (RU), in case of morphological insufficiency 0,95-1,1 Rh. Fluorescence of chlorophyll was 2.2-2.5 RU. In 326 (95.9%) indicators of metabolism, morphologic structure and function were decreased initially. Proliferation figures were 2x106-9 x107 metabolism 1 ± 0,05 RU, microcirculation 0.8-1,0 (normal 1.3-2.0) After therapy figures of metabolism, proliferation and blood circulation came back to normal in 296 (87,0%) of patients, got better in 26 (7,6%), small changed in 18 (5,4%).